Influence of Allium sativum on Pharmacodynamics and Pharmacokinetics of Gliclazide in Normal Rabbits

Abstract

Aim: In this chapter, the influence of selected dose of Allium sativum (84 mg/1.5 kg bd.wt) on the pharmacodynamics
and pharmacokinetics of selected dose of gliclazide (5.6 mg/1.5 kg bd.wt) was studied in normal rabbits. Materials and
Methods: Materials required were purchased from Sai Chemicals, Visakhapatnam, India. Inbred adult Wistar rabbits
of either sex were used for the study. Gliclazide (TD) was administered orally to all the rabbits. After a wash out period
of 1 week, the same groups of animals were administered with Allium sativum (84 mg/1.5 kg bd.wt.) orally. Again after
a further washout period of 1 week, the same group was administered with A. sativum (84 mg/1.5 kg bd.wt.) orally,
30 min before the administration of gliclazide (5.6 mg/1.5 kg bd.wt). Blood samples were withdrawn at 0, 1, 2, 3, 4, 6,
8, 12, 16, 20, and 24 h intervals from marginal ear vein puncture and were analyzed for blood glucose by GOD/POD
method in all the experiments and serum gliclazide. Results: A. sativum has found to enhance the hypoglycemic effect
of gliclazide. There was a considerable percent blood glucose reduction with gliclazide (5.6 mg/1.5 kg bd.wt) before
and after treatment with Allium sativum (104 mg/kg) in rabbits.Conclusion: The interaction between A. sativum and
gliclazide appears to be pharmacodynamic and pharmacokinetic in nature.