Anticariogenic Activity of Steroid Phytocompound Isolated from Acmella calva (DC.) R.K. Jansen

Abstract

Aim: The phytocompound Stigmast – 5 – En - 3 Beta – Ol isolated and characterized through spectral
studies was analyzed for its anticariogenic activity through disc diffusion method and in silico analysis.
Materials and Methods: Anticariogenic activity was carried against dental caries causing microbes such
as Streptococcus mutans, Streptococcus salivarius, Streptococcus sobrinus, and Staphylococcus aureus at
different concentration (20, 40, 60, 80, 100,120, 140, and 160 as robes such as Autonomous), Affiliated to
Bharathidasan University, Tiruchirappalli against S. mutans. In in silico analysis, the molecular docking of
phytocompound was analyzed against eight proteins of four pathogens which were retrieved from PDB database.
Results and Discussion: The phytocompound Stigmast – 5 – En - 3 Beta – Ol showed good response against
all proteins. Among these, the highest ligand and protein interaction was noticed in 3AIE protein of S. mutans.
The steroid compound was tested for its drug likeness and absorption, distribution, excertion, metabolism, and
toxicology properties. The results revealed that the compound accepted the three rule out of four in Lipinski
rule of five. ADME properties and toxicity of the phytocompound showed oral bioavailability which are under
permissible limit. Conclusion: From the present investigation, it was proved that the phytocompound Stigmast –
5 – En - 3 Beta – Ol is harmless and effect potent against dental caries causing microbes.