@article{Jadhav_2021, title={Piperine-hydroxy acid-cyclodextrin inclusion complexes; antioxidant, antiinflammatory, and stability studies: PART II}, volume={15}, url={http://asiapharmaceutics.info/index.php/ajp/article/view/3970}, DOI={10.22377/ajp.v15i1.3970}, abstractNote={<p>Introduction: Piperine (PIP) is a natural ingredient possessing important biological activities. However, its<br />practical usefulness is limited due to its low water solubility. In our previous research article (PART A), we<br />demonstrated inclusion complexation of PIP with cyclodextrins (CDs) in the influence of certain hydroxy acids<br />resulted in tremendous improvement in physicochemical characteristics of PIP. The aim of current research<br />work was to study biological properties and stability analysis of PIP and its lyophilized inclusion complexes.<br />Materials and Methods: Initially, Job’s plot experiment was carried out to assess the type of solubility and<br />stoichiometry of complexes. The solid inclusion complexes were obtained by lyophilization and characterized<br />by differential scanning calorimetry (DSC), X-ray powder diffractometry (XRPD), saturation solubility, in-vitro<br />dissolution, in-vitro antioxidant activity, and in-vivo anti-inflammatory activity in carrageenan-induced rat paw<br />edema model. The short-term stability studies were carried out as per the ICH guidelines. Results: During<br />assessment, the complexes performed better in terms of in-vitro antioxidant and in-vivo anti-inflammatory activities<br />than the native PIP. However, PIP: Hydroxypropyl β-CD (HPβCD): Ascorbic acid (AA) ternary complexes elicited<br />immediate and maximum onset of anti-inflammatory action, as compared to other test samples. In the stability<br />studies, no noteworthy changes were recorded concerning DSC, XRPD, and in-vitro dissolution studies over a<br />period of 6 months except complexes with HPβCD. Conclusion: Taking everything into account, complexation<br />of PIP with CDs, in the influence of AA, would be a successful way to improve its biological properties.</p>}, number={1}, journal={Asian Journal of Pharmaceutics (AJP)}, author={Jadhav, Priyanka}, year={2021}, month={Apr.} }