@article{Zanwar_2022, title={Quantitative Assessment of Luliconazole and Gallic Acid Simultaneously in Formulated Emulgel by UV Spectrophotometric Methods}, volume={16}, url={http://asiapharmaceutics.info/index.php/ajp/article/view/4394}, DOI={10.22377/ajp.v16i2.4394}, abstractNote={<p>Introduction: Three new UV spectrophotometric approaches specifically, simultaneous equation (SME),<br>absorbance ratio (ASR), and first derivative (zero crossing) spectroscopic techniques were developed and validated<br>for simultaneous estimation of luliconazole (LCZ) and gallic acid (GLA) in emulgel formulation which were<br>simple, sensitive, precise, and accurate. Materials and Methods: In SME method, absorbance was measured at<br>299 and 259 nm for both the drugs. LCZ and GLA were estimated at 299 and 266 nm in the ASR method. First<br>derivative (zero crossing) method depended on the change of UV spectra in to first derivative spectra followed<br>by measurement of first derivative signal at 249 and 259 nm for LCZ and GLA, respectively, using 4 nm as<br>wavelength interval (Δλ) and 4 as scaling factor. Developed methods were validated according to ICH guidelines<br>including parameters, namely, specificity, linearity, range, precision and accuracy, limit of detection, and limit<br>of quantification. Results and Discussion: All three techniques showed direct relation of absorbance in the<br>concentration range of 1-30 μg/ml for both the drugs. Good repeatability, low intra and inter-day variability,<br>indicate that precise agreement within the value. Recovery studies for both drugs ranged from 97 to 102 percent,<br>recommending that the methods are effective. Results of method validation parameters such as linearity and range,<br>precision, and accuracy adhere to ICH guideline acceptable limit. Conclusion: All the developed methods were<br>found to be quick, profoundly accurate and financially effective; and henceforth can be valuable for simultaneous<br>estimation of LCZ and GLA in emulgel formulation for routine quality control analysis.</p>}, number={2}, journal={Asian Journal of Pharmaceutics (AJP)}, author={Zanwar, Aarti S.}, year={2022}, month={Jun.} }