TY - JOUR AU - Lanjhiyana, S. K. PY - 2021/04/05 Y2 - 2024/03/29 TI - Colon Targeting of 5-fluorouracil Loaded Dual Cross-linked Multiparticulate System: In vitro and in vivo Characterizations JF - Asian Journal of Pharmaceutics (AJP) JA - AJP VL - 15 IS - 1 SE - ORIGINAL ARTICLES DO - 10.22377/ajp.v15i1.3964 UR - http://asiapharmaceutics.info/index.php/ajp/article/view/3964 SP - AB - <p>Objective: The present study aimed to develop sustained released 5-fluorouracil loaded chitosan-pectin blended<br />dual cross-linked gel beads system. Materials and Methods: Dual cross-linked beads were evaluated for drug<br />content, particle size, swelling degree, scanning elecron microscopy, differential scanning calorimetry, X-ray<br />diffraction, etc., for its suitability for colon targeting. Results: The developed systems were appreciably performed<br />during in vitro drug releases in simulated gastric (simulated gastric fluid) at pH 1.2, intestinal (simulated intestinal<br />fluid) at pH 6.8, and colonic fluids at pH 7.4 (simulated colonic fluid [SCF]) with and without rat cecal content<br />medium for up to 24 h. Batch formulations were shown lesser releases in acidic dissolution medium, whereas<br />augmented releases in alkaline medium at the end of 24 h studies. It was found with significant drug releases<br />(P &gt; 0.05) in SCF containing 2 and 4% w/v rat cecal as compared to control studies. During curve fittings using<br />several models, the R2 value of Higuchi matrix model confirmed for drug release was followed with anomalous<br />non-Fickian transport mechanisms. Those dual cross-linked gel beads confirmed for its improved mechanical<br />core strength, controlled, and sustained release potentials during the experimental. Gamma scintigraphic imaging<br />during in vivo studies confirms for targeting potential of optimized formulations for colon-specific region. It was<br />evident that the ionic gelation based dual cross-linked chitosan-pectin beads with divalents Ca2+ and SO4<br />-2 ions<br />exhibited better delayed drug release pattern than single cross-linked beads for colon targeting. Conclusion: The<br />prepared dual ionic cross-linked optimized formulations may be potential system for targeting drug to colon for<br />colorectal cancer.</p> ER -