TY - JOUR AU - Vaibhavi, B. Thacker PY - 2021/04/05 Y2 - 2024/03/28 TI - Protective Effect of Polyherbal Composition against Hemorrhagic Shock Condition in an Experimental Rat Model JF - Asian Journal of Pharmaceutics (AJP) JA - AJP VL - 15 IS - 1 SE - ORIGINAL ARTICLES DO - 10.22377/ajp.v15i1.3971 UR - http://asiapharmaceutics.info/index.php/ajp/article/view/3971 SP - AB - <p>Objective: Hemorrhagic shock (HS) is a condition which requires an immediate care and if not treated on time can<br />result in death. Hence, we aim to study the protective role of fixed dose combination of polyherbal composition<br />comprising of an equal amount of aqueous extracts of Azadirachta indica leaves, Carica papaya leaves, and<br />methanolic extract of Andrographis paniculata against fixed volume bleed HS model. Materials and Methods: The<br />study was conducted with 48 male and 48 female Wistar rats divided into 8 groups, normal control (G1), disease<br />control (sublethal and lethal, G2 and G3) and 5 treatment groups (G4–G8). Treatment was initiated on the same<br />day and continued for 14 days. At the end of 14 days, hemorrhage was induced by withdrawing 40% of circulatory<br />blood volume for all groups and 60% of circulatory blood volume for G3. Treatment was continued further for<br />7 days. Complete blood count, serum levels of glucose, sodium, potassium, and rectal temperature were measured<br />at before, 3 h, 24 h, 72 h, and 7 days post-hemorrhage, whereas interleukin (IL)-6, nuclear factor kappa B (NFƘB),<br />and vasopressin levels were measured at 24 h time point. Serum lactate and bicarbonate levels were measured<br />at 3 h and at the end of the study. Statistical analysis was done using one way ANOVA followed by Dunnett’s<br />test. P &lt; 0.05 was considered statistically significant. Results: At 24 h, glucose levels were high and hematocrit<br />levels were low compared to NC group, which were recovered to normal in all treatment groups at the end of the<br />experiment except in disease control group. Our findings were further supported by serum level of electrolytes<br />as well as IL-6, NF-ƘB, and vasopressin levels which were elevated in response to HS condition at 24 h posthemorrhage.<br />We have seen significant (P &lt; 0.0001, P &lt; 0.001, P &lt; 0.01, P &lt; 0.05) changes in the level of these<br />parameters in disease control group compared to other groups. Recovery at the end of the experiment indicates<br />that animals have responded to our treatments with maximum effect has been seen in G7 group. Conclusion: The<br />polyherbal extracts used in this experiment have the potential to be used alone or in combination with available<br />conventional medical therapy against HS condition to provide symptomatic relief, to improve quality of life and<br />to promote the survival.</p> ER -