TY - JOUR AU - Dharmamoorthy, Dr. G. PY - 2022/09/15 Y2 - 2024/03/28 TI - Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Cedazuridine and Decitabine by in Bulk and its Pharmaceutical Dosage Form JF - Asian Journal of Pharmaceutics (AJP) JA - AJP VL - 16 IS - 3 SE - ORIGINAL ARTICLES DO - 10.22377/ajp.v16i3.4478 UR - http://asiapharmaceutics.info/index.php/ajp/article/view/4478 SP - AB - <p>Objective: A new sensitive accurate and precise reverse-phase high-performance liquid chromatography<br>(RP-HPLC) method was developed for the simultaneous estimation of cedazuridine and decitabine in bulk and<br>pharmaceutical formulation. Materials and Methods: Chromatographic separation was achieved through Altima<br>C18 column (4.6*150 nm, 5 μm) using 0.01 Kh2PO4:acetonitrile (60:40 v/v) mixture used as the mobile phase.<br>The Waters 2695, Reciprocating Water-510 pump system with PDA detector, and EMPOWERPRO software<br>were monitored at detection wavelength 257 nm on flow rate 1 mL/min and the method was validated as per<br>ICH guidelines (ICH,Q2 [R1]). Results and Discussion: Cedazuridine and decitabine were eluted at 2.248 min<br>and 2.956 min, respectively, with good resolution. Plate count and tailing factor were very satisfactory, so this<br>method was optimized and to be validated. Conclusion: This RP-HPLC method was successfully applied for the<br>simultaneous determination of cedazuridine and decitabine in their pharmaceutical formulation and, hence, can be<br>used for the routine analysis of these drugs in combined dosage form.</p> ER -