TY - JOUR AU - Soni, Vandana PY - 2022/12/15 Y2 - 2024/03/29 TI - Mucoadhesive Film for Local Delivery to Oral Cancer: Formulation Development, Box–Behnken Experimental Design, and In vitro Characterization JF - Asian Journal of Pharmaceutics (AJP) JA - AJP VL - 16 IS - 4 SE - ORIGINAL ARTICLES DO - 10.22377/ajp.v16i4.4600 UR - http://asiapharmaceutics.info/index.php/ajp/article/view/4600 SP - AB - <p>Background: Oral cancer affects millions of people worldwide, which is more common in those 35 and older.<br>Low-targeted therapy options for oral malignancies and poor drug uptake by lesions in the oral cavity followed<br>by systemic injection contribute to high mortality and poor patient quality of life. Aim: The present study was<br>undertaken for optimization and assessment of the designed cisplatin mucoadhesive film made of hydroxyl propyl<br>methyl cellulose and chitosan that can be used locally to treat oral cancer. Materials and Methods: The films were<br>prepared by solvent casting method, employing a Box–Behnken design, and subsequently confirmed by ANOVA<br>analysis. The formulations were optimized by swelling index and residence time. Physiochemical characteristics<br>of oral films, including pH, weight, thickness, tensile strength, folding endurance, and in vitro drug release, were<br>also assessed. Results and Discussion: According to the factors selected for the optimization, the formulation<br>(F3) was selected as the optimized formulation with highest desirability of 0.997. In vitro release profile indicated<br>initial burst release and subsequently a sustained release of cisplatin from the film for 24 h. The release kinetics<br>data displayed a Korsmeyer–Peppas release model with the best-fit match R2 value (0.9257). In vitro exposing the<br>KB-3-1 cell line to the optimized film resulted in dose-dependent cancer cell death. The IC50 of free cisplatin and<br>cisplatin mucoadhesive film (F3) on KB-3-1 was found to be 94.25 μg/ml and 23.64 μg/ml, respectively. This study<br>demonstrates that local bioadhesive therapies are effective in treating cancer of the oral cavity. Conclusion: On the<br>basis of data, it could be concluded that the number of polymers used was the critical factor for the production of<br>cisplatin mucoadhesive film that had a substantial influence on their physical attributes. This factorial design study<br>has served as a valuable tool for optimizing mucoadhesive film for the delivery of cisplatin.</p> ER -