TY - JOUR AU - Singh, Manisha PY - 2019/08/31 Y2 - 2024/03/29 TI - Fabrication, Validation, and Stability Analysis of Melaleuca alternifolia Oil-in-water Microemulsion for Improved Transdermal Application JF - Asian Journal of Pharmaceutics (AJP) JA - AJP VL - 13 IS - 3 SE - ORIGINAL ARTICLES DO - 10.22377/ajp.v13i3.3333 UR - https://asiapharmaceutics.info/index.php/ajp/article/view/3333 SP - AB - <p>Aim: The aim of the study was to develop oil-in-water microemulsion (ME) system for Melaleuca alternifolia<br />for targeting skin disorders. Materials and Methods: The formulation of ME system was done through the water<br />titration method, which involved various oils, water, surfactants, and cosurfactants. The existence zones of ME<br />were determined by constructing ternary phase diagrams, where surfactants and cosurfactants (Smix) were taken in<br />different ratios along with different combinations of oil and Smix. Quantitative estimation of tea tree oil (TTO) was<br />performed using gas chromatography-mass spectrometry followed by total terpenoid estimation, and thereafter,<br />solubility studies of TTO were performed using various excipients. TTO-ME was fabricated and characterized<br />using modern characterization techniques (DLS, transmission electron microscopy, and Fourier transform infrared)<br />along with statistical validation and in vitro drug release assessment. Results and Discussion: The optimized<br />TTO loaded MEs (TTO ME) showed the particle size range between 32 and 160 nm with poly dispersibility index<br />of 0.297 and zeta potential of −3.19 mv with almost spherical morphology. The optimized formulation showed<br />the sustained release of the therapeutic compound and was found to be stable at room temperature for 1 year,<br />suggesting being a promising formulation against dermal infections. Conclusion: TTO was successfully loaded<br />in thermodynamically stable ME system which enhanced its permeation through the transdermal route. Therefore,<br />a novel drug delivery system was introduced, which can be studied on various in vitro and in vivo models further<br />to establish its better therapeutic efficacy for various skin disorders.</p> ER -