TY - JOUR AU - Prarthana, Dr. J. PY - 2021/11/01 Y2 - 2024/03/28 TI - Isolation and Characterization of Potential Microorganism from Dhanyamla - An Ayurvedic Formulation with Therapeutic Properties JF - Asian Journal of Pharmaceutics (AJP) JA - AJP VL - 15 IS - 3 SE - ORIGINAL ARTICLES DO - 10.22377/ajp.v15i3.4155 UR - https://asiapharmaceutics.info/index.php/ajp/article/view/4155 SP - AB - <p>Objectives: Ayurveda is one of the oldest codified medical knowledge systems with equal emphasis on curative,<br />preventive, and promotive aspects of health. Ayurveda pharmaceutics was developed from the quest to administer<br />plants, animals, or metals and minerals products in a palatable and longer shelf life modification. The study<br />aims to evaluate and characterize the microbial sp. and their biochemical properties in extrapolating its use<br />as a potent probiotic formulation with multifaceted use. Materials and Methods: The microbial diversity of<br />the formulation was evaluated by isolation followed by its microbial characterization using Gram’s staining,<br />biochemical characterization using catalase assay, and molecular characterization by sequencing the internal<br />transcribed spacer (ITS) region. Results: The study revealed that the bacteria isolated in the present study were<br />Gram-positive, rod-shaped organism that exhibited catalase-positive test. Further, molecular characterization<br />studies using the ITS sequence analysis revealed that the isolated organism showed similarity with that of Bacillus<br />species. Conclusion: Therapeutic efficacy of any formulation depends on the process of its preparation, the kind<br />of microflora that is established during aging or fermentation, and the kind of bioactive compounds released<br />during fermentation. The present study identifies the microorganism that plays a pivotal role in this fermentation<br />process and renders therapeutic properties to Dhanyamala formulation. This study can form the basis for further<br />investigations on formulating this as a promising probiotic supplement.</p> ER -