TY - JOUR AU - Gaikwad, Rahul Prabhu PY - 2023/03/15 Y2 - 2024/03/29 TI - Molecular Docking Studies of Phytochemicals from Five Medicinal Plants against Resistance genes Protein isolated from MDR Salmonella Typhimurium JF - Asian Journal of Pharmaceutics (AJP) JA - AJP VL - 17 IS - 1 SE - ORIGINAL ARTICLES DO - 10.22377/ajp.v17i1.4725 UR - https://asiapharmaceutics.info/index.php/ajp/article/view/4725 SP - AB - <p>Introduction: In the present study, the antibacterial activity against multidrug-resistant (MDR) Salmonella<br>Typhimurium of different extracts of Five Medicinal Plants was investigated along with their phytochemical<br>analysis by gas chromatography-mass spectrometry (GC-MS) and Molecular Docking Studies of Phytochemicals<br>to explore antimicrobial compounds present in extracts. Materials and Methods: The antibacterial activities of<br>different extracts of Five Medicinal Plants were evaluated against isolated MDR S. Typhimurium by disc diffusion<br>method. Docking studies were carried out using different required docking platforms along with phytochemical<br>analysis by GC-MS. Results and Discussion: The phytochemical analysis by quantitative and qualitative methods<br>of the extract of Allium sativum, Bridelia micrantha, Citrus lemon, Glycyrrhiza glabra, and Punica granatum<br>showed presence of amino acids, alkaloids, glycosides, saponins, flavonoids, steroids, and terpenoids. It was<br>discovered that crude extracts of these plants worked well against MDR S. Typhimurium. The zone of inhibition<br>values for the crude extract of A. sativum, B. micrantha, C. lemon, G. glabra, and P. granatum was found to be,<br>10.12 ± 1.71 mm, 23.50 ± 0.00 mm, 26.60 ± 0.64 mm, 29.20 ± 0.11 mm, and 19.50 ± 0.00 mm, respectively.<br>Docking results of phyto-ligand with selected resistance genes proteins reveals that the binding energy ranges<br>from −2.9 to −9.2 kcal/moL. G. glabra roots (Ligand 6-Androstanone, 3-(3, 4-dimethylphenyl)-3-methyl) showed<br>the lowest binding energy of −9.2 kcal/moL. Conclusion: To produce broad-spectrum antimicrobial compounds,<br>it is still important to assess the antibacterial potential of medicinal plants. Traditional medicine is one of the<br>most generally available types of treatment in disadvantaged countries. According to the study’s findings,<br>phytochemicals from five medicinal plants extract have in vitro antibacterial activity along with phyto-ligand<br>docking analysis providing early evidence that the plants may be used to treat MDR infections.</p> ER -