Influence of the Novel Anticancer Agents on the Activity of Outward Rectifier Potassium Currents in Human Prostate Cancer Cell Line - LNCaP

Abstract

Background: Phenolic compounds are ubiquitous in dietary fruits and vegetables and have been proposed to have antioxidant, antibacterial, anti-inflammatory, and anticancer effects. Voltage gated K+ channels (IK) contribute to various cellular mechanisms involved in the tumor cell progression. Therefore, the modulation of IK current may be a potential target for cancer therapy. In this study, we electrophysiologically characterized four phenolic compounds for their modulatory activities on IK currents. Materials and Methods: The whole cell configuration of the patch clamp recording technique was used to record the IK currents from human prostate cancer line (LNCaP). In addition, 200 μM of phenolic compounds (curcumin, resveratrol, rutin, and troxerutin) were externally perfused to the cells. Results: Obtained results provide evidence that resveratrol at 200 μM inhibited more than half of the IK current amplitude. On the other hand, curcumin, rutin, and troxerutin did not show any modulatory effect on IK current in LNCaP cells. Conclusion: This study demonstrates that not all the anticancer compounds are effective inhibitors of IK current, but only few of them. The IK current inhibitors might exhibit effective antimetastatic properties.