Quantification of Arbidol by RP-HPLC with photo diode array detection

Main Article Content

Narendra Angirekula


Introduction: A new liquid chromatographic method has been developed for the determination of Arbidol in capsules. Arbidol is the brand name for the anti-viral drug Umifenovir. Umifenovir is used for the treatment for influenza infections. Materials and Methods: SHIMADZU HPLC system with Agilent column (150 mm × 4.6 mm i.d., 3.5 μm particle size) was used for the chromatographic study of Umifenovir on isocratic mode. A mixture of 10 mM tetra butyl ammonium hydrogen sulphate and acetonitrile (50: 50, v/v) was used as mobile phase with flow rate 0.8 mL/min. for the chromatographic separation of Umifenovir. PDA detector was used (UV detection: at 223 nm) and the total run time was 10 mins. Results and Discussion: Umifenovir has obeyed Beer-Lambert’s law 0.5-50 μg/mL with correlation coefficient 0.9999. The LOD and LOQ are found to be 0.1581 μg/mL and 0.4829 μg/mL respectively. Conclusions: The method is simple, precise and accurate and can be used for the determination of Umifenovir. The method was validated as per ICH guidelines.


Download data is not yet available.

Article Details

How to Cite
Angirekula, N. (2018). Quantification of Arbidol by RP-HPLC with photo diode array detection. Asian Journal of Pharmaceutics (AJP), 12(02). https://doi.org/10.22377/ajp.v12i02.2391