Reversed phase liquid chromatographic conditions for simultaneous determination of antihypertensive formulations

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Shalini Joshi
Amrita Sharma
MS Rawat
CS Bal


Rapid and simple reversed phase chromatographic conditions have been developed to separate pharmacologically
active components of antihypertensive drug formulations (enalapril maleate + amlodipine besilate and
ramipril 1 hydrochlorothiazide) on high-performance liquid chromatography (HPLC) and thin layer chromatography.
The mobile phase consists of methanol–water (60:40 v/v) for reversed-phase thin layer chromatography (RPTLC). Detection
was carried out by iodine vapors. The HPLC method was developed on a C-18 column with detection carried out by a UV
detector at 215 and 220 nm for enalapril maleate 1 amlodipine besilate and ramipril + hydrochlorothiazide, respectively.
The HPLC method was validated using data elements specificity, linearity and range, and accuracy and precision.


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Joshi, S., Sharma, A., Rawat, M., & Bal, C. (2014). Reversed phase liquid chromatographic conditions for simultaneous determination of antihypertensive formulations. Asian Journal of Pharmaceutics (AJP), 3(4).


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Joshi, et al.: Reversed phase liquid chromatographic conditions for simultaneous determination of antihypertensive formulations