Effect of Plasmid Presence and Stability on Growth of Escherichia coli DH5α with Use of Drugs, Chemicals, and Radiation

Dr. Praveen Kumar Vemuri


Objective: The purpose of the study delineates the growth and plasmid stability of E. coli DH5α host system.
Materials and Methods: Different concentrations of drugs, chemicals, and various frequency of radiations were
subjected to the host system to verify the colony-forming units along with plasmid concentration and stability.
Results: Among chemicals, acridine orange showed the highest effect on growth of DH5α, while among the
drugs, danthrone showed maximum effect on the growth of the organism. Radiofrequency of 2 GHz and lowintensity
microwave radiation were recorded as the highest inhibitory effects. However, there is no significant
effect in growth observed in exposure to UV rays. Conclusion: The present work discussed that drugs, chemicals,
radiofrequency, and microwave radiation have a huge effect on the growth of organism and also on the concentration
and stability of plasmid.

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DOI: http://dx.doi.org/10.22377/ajp.v13i3.3294


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