Mucoadhesive nanoparticles from tamarind seed polysaccharides for sustained delivery of anticancer drug irinotecan

Pranjal Saikia, Bhanu P. Sahu, S. K. Dash

Abstract


The present study is aimed at development and optimization of mucoadhesive nanoparticles (NPs) from natural
mucoadhesive polysaccharides extracted from Tamarind seeds (Tamarindus indica) for the sustained delivery of
anticancer drug irinotecan. The drug loaded NPs were prepared by ion gelation method with the isolated polysaccharide by
homogenization followed by lyophilization. The polysaccharides were cross‑linked with sodium alginate in different ratios. The
formulations were optimized using two level factorial design (Design Expert ‑ 8.0.7.1) using the polysaccharide to alginate
ratio, homogenization time and homogenization speed as independent variables and particle size (PS), drug entrapment
efficiency and cumulative drug release as the dependent variables. The NPs were characterized in terms of PS, entrapment efficiency, drug loading (DL), in vitro drug release and cell viability studies in mice. Stable NPs were obtained with average PS of 405 ± 25.2 nm. The preparations were homogenous showing polydispersity index of 0.497 ± 0.02. The formulation showed up to 95.36 ± 3.1% (w/w) yield showing DL of 1.0 ± 0.2% (w/w). The entrapment efficiency was found to be
46.56 ± 1.5% (w/w). In vitro drug release showed initial burst release followed by controlled release pattern showing up
to 60% release in 12 h. The average cell viability was found to be 80% in case of the control group, which was reduced to
36% for NPs treated groups respectively. The Fourier transform infrared studies showed no incompatibility in the formulated
NPs. It may be concluded from the study that tamarind seed polysaccharides may be suitable for formulation of mucoadhesive NPs for better efficacy and sustained delivery of anticancer drug irinotecan with reduced toxicity.
Key words: Homogenization, irinotecan, mucoadhesive, nanoparticles, tamarind

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References


Emerson DL. Liposomal delivery of camptothecins. Pharm Sci Technolo

Today 2000;3:205‑9.

Takimoto CH, Arbuck SG. Topoisomerage I targeting agents: The

camptothecins. In: Chabner BA, longo DL, editors. Cancer Chemotherapy

and Biotherapy: Principles and Practices. 3rd ed. Philadelphia: Lippincott

Williams and Wilkins; 2001. p. 579‑646.

Cortesi R, Nastruzzi C. Liposomes, micelles and microemulsions as

new delivery systems for cytotoxic alkaloids. Pharm Sci Technolo Today

;2:288‑98.

Chow DS, Gong L, Wolfe MD, Giovanella BC. Modified lactone/carboxylate

salt equilibria in vivo by liposomal delivery of 9‑nitro‑camptothecin. Ann

N Y Acad Sci 2000;922:164‑74.

Williams J, Lansdown R, Sweitzer R, Romanowski M, LaBell R,

Ramaswami R, et al. Nanoparticle drug delivery system for intravenous

delivery of topoisomerase inhibitors. J Control Release 2003;91:167‑72.

Hattori Y, Shi L, Ding W, Koga K, Kawano K, Hakoshima M, et al. Novel

irinotecan‑loaded liposome using phytic acid with high therapeutic

efficacy for colon tumors. J Control Release 2009;136:30‑7.

Sano M, Miyata E, Tamano S, Hagiwara A, Ito N, Shirai T. Lack of

carcinogenicity of tamarind seed polysaccharide in B6C3F1 mice. Food

Chem Toxicol 1996;34:463‑7.

Burgalassi S, Panichi L, Saettone MF, Jacobsen J, Rassing MR.

Development and in vitro/in vivo testing of mucoadhesive buccal patches

releasing benzydamine and lidocaine. Int J Pharm 1996;133:1‑7.

Sumathi S, Ray AR. Release behaviour of drugs from tamarind seed

polysaccharide tablets. J Pharm Pharm Sci 2002;5:12‑8.

Kulkarni D, Ddwivedi DK, Sarin JP, Singh S. Tamarind seed

polyose: A potential polysaccharide for sustained release of verapamil

hydrochloride as a model drug. Indian J Pharm Sci 1997;59:1‑7.

Saettone MF, Burgalassi S, Giannaccini B, Boldrini E, Bianchini P,

Luciani G. Ophthalmic solutions viscosified with tamarind seed

polysaccharide. International Patent Application Number . PCT Int Appl

WO 97 28,787, 1997.

Ghelardi E, Tavanti A, Celandroni F, Lupetti A, Blandizzi C, Boldrini E,

et al. Effect of a novel mucoadhesive polysaccharide obtained from

tamarind seeds on the intraocular penetration of gentamicin and

ofloxacin in rabbits. J Antimicrob Chemother 2000;46:831‑4.

Sahoo R, Sahoo S, Nayak PL. Release behavior of anticancer drug

paclitaxel from tamarind seed polysaccharide galactoxyloglucan. Eur J

Sci Res 2010;47:197‑206.

Uccello‑Barretta G, Nazzi S, Balzano F, Di Colo G, Zambito Y, Zaino C, et al.

Enhanced affinity of ketotifen toward tamarind seed polysaccharide in

comparison with hydroxyethylcellulose and hyaluronic acid: A nuclear

magnetic resonance investigation. Bioorg Med Chem 2008;16:7371‑6.

Sangeetha S, Harish G, Samanta MK. chitosan-based nanospheres as

drug delivery system for cytarabine. Int J Pharm Bio Sci 2010;1:1-8

Fattal E, Rojas J, Roblot‑Treupel L, Andremont A, Couvreur P. Ampicillin‑loaded

liposomes and nanoparticles: Comparison of drug loading, drug release

and in vitro antimicrobial activity. J Microencapsul 1991;8:29‑36.

Nair R, Vishnu Priya K, Arun Kumar KS, Badivaddin T, Sevukarajan M.

Formulation and evaluation of solid lipid nanoparticles of water soluble

drug: Isoniazid. J Pharm Sci Res 2011;3:1256‑64.

Shokri N, Akbari Javar H, Fouladdel Sh, Khalaj A, Khoshayand M,

Dinarvand R, et al. Preparation and evaluation of poly (caprolactone

fumarate) nanoparticles containing doxorubicin HCI. Daru 2011;19:12‑22.

Ramsay E, Alnajim J, Anantha M, Zastre J, Yan H, Webb M, et al. A novel

liposomal irinotecan formulation with significant anti‑tumour activity: Use

of the divalent cation ionophore A23187 and copper‑containing liposomes

to improve drug retention. Eur J Pharm Biopharm 2008;68:607‑17.

Mielcarek J, Czernielewska A, Czarczynska B. Inclusion complexes of

felodipine and amlodipine with methyl‑b‑cyclodextrin. J Incl Phenom

Macrocycl Chem 2006;54:17‑21.




DOI: http://dx.doi.org/10.22377/ajp.v7i4.338

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