Preparation and evaluation of hand rub disinfectant

Main Article Content

Amol Padsalg
Devendra Jain
Sanjay Bidkar
Dommati Harinarayana
Vijay Jadhav


The present work is to formulate effective hand rub disinfectant by selecting broad-spectrum antimicrobial and antifungal agents. Increased need of easy-to-use hand rub disinfectant in pharmaceutical, food processing industry, hospitals, and in clinical labs necessitated development of the best possible product. Formulations were prepared using Benzalkonium chloride, chlorhexidine gluconate as potent disinfectants along with alcohol 70%. In low concentration they were highly effective without leaving any toxic effect on the user’s skin. A synergistic effect was observed when ethyl alcohol 70% was used in combination. Suitable emollient and skin conditioning agents were used to avoid possible dehydrating effect on the user’s skin. Excellent volatility of the preparation was observed after use, leaving a thin antimicrobial film on the user’s hand.
The use of suitable excipient brought effective removal of after the use leaving a soothening effect on the skin. The aim of present work is to develop hand rub formulation which evaporates quickly after applications leaving no trace on hands as in case of hand rub gels. The present formulation was found to be effective when compared with marketed liquid hand rub.


Download data is not yet available.

Article Details

How to Cite
Padsalg, A., Jain, D., Bidkar, S., Harinarayana, D., & Jadhav, V. (2014). Preparation and evaluation of hand rub disinfectant. Asian Journal of Pharmaceutics (AJP), 2(1).


Zaragoza M, Sallés M, Gomez J, Bayas JM, Trilla A. Handwashing

with soap or alcoholic solutions: A randomized clinical trial of its

effectiveness. Am J Infect Control 1999;27:258-61.

Rotter M. Hand washing and hand disinfection. In: Mayhall CG, Hospital

epidemiology and infection control. 2nd ed. Philadelphia, PA: Lippincott

Williams and Wilkins; 1999;312-5.

Larson E. Guideline for use of topical antimicrobial agents. Am J Infect

Control 1988;16:253-66.

Fox MK, Langner SB, Wells RW. How good are hand washing practices?

Am J Nursing 1974;74:1676-8.

Rotter ML. Hygienic hand disinfection. Infect Control 1984;1:18-22.

Kramer A, Rudolph P, Kampf G, Pittet D. Limited efficacy of alcohol-

based hand gels. Lancet 2002;359:1489-90.

Larson EL, Eke PI, Laughon BE. Efficacy of alcohol-based hand rinses

under frequent-use conditions. Antimicrob Agents Chemother


Larson EL; APIC Guidelines Committee. APIC guideline for hand

washing and hand antisepsis in health care settings. Am J Infect Control


Boyce JM, Kelliher S, Vallande N. Skin irritation and dryness associated

with two hand-hygiene regimens: Soap-and-water hand washing

versus hand antisepsis with an alcoholic hand gel. Infect Control Hosp

Epidemiol 2000;21:42-8.

Berman RE, Knight RA. Evaluation of hand antisepsis. Arch Environ

Health 1969;18:781-3.

Mackintosh CA, Hoffman PN. An extended model for transfer of micro-

organisms via the hands: Differences between organisms and the effect

of alcohol disinfection. J Hyg (Lond) 1984;92:345-55.

Ulrich JA. Clinical study comparing hibistat (0.5% chlorhexidine

gluconate in 70% isopropyl alcohol) and betadine surgical scrub (7.5%

povidone-iodine) for efficacy against experimental contamination of

human skin. Curr Ther Res 1982;31:27-30.

Widmer AF, Replace hand washing with use of a waterless alcohol hand

rub. Clin Infect Dis 2000;31:136-43.

Harrington C, Walker H. The germicidal action of alcohol. Boston Med

Surg J 1903;148:548-52.

Larson EL, Eke PI, Laughon BE. Efficacy of alcohol-based hand rinses

under frequent-use conditions. Antimicrob Agents Chemother


Most read articles by the same author(s)