Novel HPLC analysis of cefadroxil in bulk formulation
Main Article Content
(first generation) in bulk form. An Inertsil ODS, 4.60 Ã— 250 mm2, 5 Î¼m analytical column was used with an eluting
system consisting of a mixture of phosphate buffer (pH 6.5)-methanol 78-32% (v/v) at a flow-rate 1.5 ml/min. Detection
was performed by UV-Vis detector at 210 nm, resulting in limit of detection of 0.06 ppm for cefadroxil per 20-Î¼l injection.
A linear relationship was observed up to 0.2 ppm for cefadroxil. Analysis time was less than 10 min. The statistical evaluation
of the method was examined by means of within-day repeatability (n = 6) and day-to-day precision (n = 7) and was found to be satisfactory with high accuracy and precision. The method may be applied for the determination of the cefadroxil in
bulk formulation in future.
Key words: Cefadroxil, cephalosporins, HPLC, phosphate buffer
This is an Open Access article distributed under the terms of the Attribution-Noncommercial 4.0 International License [CC BY-NC 4.0], which requires that reusers give credit to the creator. It allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, for noncommercial purposes only.
Eliopoulos GM, Reiszner E, Wennersten C, Moellering RC Jr. In vitro
activity of BMY-28100, a new oral cephalosporin. Antimicrob Agents
Chin N, Neu HC. In vitro activity of BMY-28100, a new oral cephalosporin.
Antimicrob Agents Chemother 1987;31:480-3.
Yun E, Prince A, McMillin J, Welch L. High-performance liquid
chromatographic separation and electrochemical detection of
cephalosporins J.Chromatogr 1998;B712:145.
El-Gindy A, El Walily A, Bedair M. First-derivative spectrophotometric
and LC determination of cefuroxime and cefadroxil in urine. J Pharm
Biomed Anal 2000;23:341-52.
Gallo-Martinez L, Sevillano-Cabeza A, Campins-Falco P, Bosch-Reig F. A
new derivatization procedure for the determination of cephalexin with
,2-naphthoquinone 4-sulphonate in pharmaceutical and urine samples
using solid-phase extraction cartridges and UV-visible detection, Anal
Chim Acta 1998;370:115.
Jehl F, Birckel P, Monteil H. Hospital routine analysis of penicillins,
third-generation cephalosporins and aztreonam by conventional and high-speed high-performance liquid chromatography. J Chromatogr
Fabre H, Blanchin MD, Kok W. Liquid chromatography with amperometric
detection for the determination of cephalosporins in biological fluids.
Ting S. Reverse-phase liquid chromatographic analysis of cephalosporins.
J Assoc Off Anal Chem 1988;71:1123-30.
Moore CM, Sato K, Katsumata Y. High-performance liquid
chromatographic determination of cephalosporin antibioti cs using
3 mm ID columns. J Chromatogr 1991;539:215-20.
Abdel-Hamid M FSQ spectrophotometric and HPLC analysis of some
cephalosporins in the presence of their alkali-induced degradation
products Farmaco 1998;53:132.
Coran S, Bambagiotti-Alberti M, Giannellini V, Baldi A, Picchioni G, Paoli
F. Development of a densitometric method for the determination of
cephalexin as an alternative to the standard HPLC procedure. J Pharm
Biomed Anal 1998;18:271-4.
Gallo MartÃnez L, CampÃns FalcÃ³ P, Sevillano Cabeza A. Comparison
of several methods used for the determination of cephalosporins.
Analysis of cephalexin in pharmaceutical samples. J Pharm Biomed
Joshi S. HPLC separation of antibiotics present in formulated and
unformulated samples. J Pharm Biomed Anal 2002;28:795-809.
ICH. Validation of analytical procedures: Methodology. Geneva:
International Conference on Harmonisation, IFPMA; 1996.