Green synthesis of silver nanoparticles using Arnebia nobilis root extract and wound healing potential of its hydrogel

Seema Garg, Amrish Chandra, Avijit Mazumder, Rupa Mazumder

Abstract


The present study reports wound healing potential of silver nanoparticles (AgNPs) hydrogel using Arnebia nobilis (A. nobilis)
root extract. It makes a convenient method for the green synthesis of AgNPs and evaluated for its wound healing activity.
Silver has been used for the treatment of medical ailments for over 100 years due to its natural antibacterial and antifungal
properties. The synthesized nanoparticles were characterized using UV‑visible spectrophotometer; transmission electron
microscopy, X‑ray diffraction (XRD), scanning electron microscopy, and Fourier transform infra‑red spectrometry. The
nanoparticles were found to be mostly spherical in shape. XRD study shows that the particles are crystalline in nature with
face centered cubic geometry. The synthesized AgNPs exhibited good antibacterial potential against both Gram‑positive and
Gram‑negative bacterial strain as measured using well diffusion assay. The recent emergence of nanotechnology has provided
a new therapeutic modality in AgNPs for use in wounds. We investigated the wound‑healing potential of AgNPs hydrogel
using A. nobilis root extract in an excision animal model. The study showed that hydrogel of AgNPs using A. nobilis root extract
exert positive effect due to their antimicrobial potential. The results provide insight into the mechanism of actions of AgNPs
and have provided a novel therapeutic direction for wound treatment in the clinical practice.
Key words: Antibacterial potential, Arnebia nobilis, Fourier transform infra‑red, silver nanoparticles, wound healing, X‑ray diffraction

Full Text:

PDF

References


Glom RW. Functionalized nanoparticles for applications in biotechnology.

J Dispers Sci Technol 2005;26:389‑14.

Chan WC. Bionanotechnology progress and advances. Biol Blood

Marrow Transplant 2006;12:87‑91.

Boisselier E, Astruc D. Gold nanoparticles in nanomedicine:

Preparations, imaging, diagnostics, therapies and toxicity. Chem Soc

Rev 2009;38:1759‑82.

Staii C, Johnson AT Jr, Chen M, Gelperin A. DNA‑decorated carbon

nanotubes for chemical sensing. Nano Lett 2005;5:1774‑8.

Gardea‑Torresdey JL, Parsons JG, Dokken K, Peralta‑Videa JR, Troiani H,

Santiago P, et al. Formation and growth of Au nanoparticles inside live

alfalfa plants. Nano Lett 2002;2:397‑401.

Gardea‑Torresdey JL, Gomez E, Peralta‑Videa JR, Parsons JG, Troiani H,

Jose‑Yacaman M. Alfalfa sprouts: A natural source for the synthesis of

silver nanoparticles. Langmuir 2003;19:1357‑61.

Saxena A, Tripathi RM, Singh RP. Biological synthesis of silver

nanoparticles by using onion (Allium cepa) extract and their antibacterial

activity. Dig J Nanomater Biostructure 2010;5:427‑32.

Kumar V, Yadav SC, Yadav SK. Syzygium cumini leaf and seed extract

mediated biosynthesis of silver nanoparticles and their characterization.

J Chem Technol Biotechnol 2010;85:1301‑9.

Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, et al.

Rapid synthesis of silver nanoparticles using dried medicinal plant of

basil. Colloids Surf B Biointerfaces 2010;81:81‑6.

Garg S, Chandra A, Mazumder A, Mazumder R. Analgesic potential of

hydrogels of silver nanoparticles using aqueous extract of Saraca indica

bark. Int J Pharm Sci Res 2014;5:240‑5.

Garg S, Chandra A. Biosynthesis and anthelmintic activity of silver nano

particles using Saraca indica leaves extract. Int J Ther Appl 2012;7:9‑12.

Garg S. Microwave‑assisted rapid green synthesis of silver nanoparticles

using Saraca indica leaf extract and their antibacterial potential. Int J

Pharm Sci Res 2013;4:3615‑9.

Garg S. Rapid biogenic synthesis of silver nano particles using black

pepper (Piper nigrum) corn extract. Int J Innov Biol Sci 2012;3:5‑10.

Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, et al. Antimicrobial effects

of silver nanoparticles. Nanomedicine 2007;3:95‑101.

Brandt O, Mildner M, Egger AE, Groessl M, Rix U, Posch M, et al.

Nanoscalic silver possesses broad‑spectrum antimicrobial activities

and exhibits fewer toxicological side effects than silver sulfadiazine.

Nanomedicine 2012;8:478‑88.

Mohanty S, Mishra S, Jena P, Jacob B, Sarkar B, Sonawane A. An

investigation on the antibacterial, cytotoxic, and antibiofilm efficacy

of starch‑stabilized silver nanoparticles. Nanomedicine 2012;8:916‑24.

Nanda A, Saravanan M. Biosynthesis of silver nanoparticles from

Staphylococcus aureus and its antimicrobial activity against MRSA and

MRSE. Nanomedicine 2009;5:452‑6.

Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M. Fungus‑mediated

synthesis of silver nanoparticles and their activity against pathogenic

fungi in combination with fluconazole. Nanomedicine 2009;5:382‑6.

Fayaz AM, Balaji K, Girilal M, Yadav R, Kalaichelvan PT, Venketesan R.

Biogenic synthesis of silver nanoparticles and their synergistic effect

with antibiotics: A study against gram‑positive and gram‑negative

bacteria. Nanomedicine 2010;6:103‑9.

Dar MA, Ingle A, Rai M. Enhanced antimicrobial activity of silver

nanoparticles synthesized by Cryphonectria sp. evaluated singly and

in combination with antibiotics. Nanomedicine 2013;9:105‑10.

Strydom SJ, Rose WE, Otto DP, Liebenberg W, de Villiers MM. Poly

(amidoamine) dendrimer‑mediated synthesis and stabilization of

silver sulfonamide nanoparticles with increased antibacterial activity.

Nanomedicine 2013;9:85‑93.

Nadworny PL, Wang J, Tredget EE, Burrell RE. Anti‑inflammatory

activity of nanocrystalline silver in a porcine contact dermatitis model.

Nanomedicine 2008;4:241‑51.

Kwan KH, Liu X, To MK, Yeung KW, Ho CM, Wong KK. Modulation of

collagen alignment by silver nanoparticles results in better mechanical

properties in wound healing. Nanomedicine 2011;7:497‑504.

Arora A, Rastogi D, Gupta D, Gulrajani ML. Kinetics and thermodynamics

of dye extracted from Arnebia nobilis Rech f. on wool. Indian J Fibre Text

Res 2012;37:91‑7.

Arora A, Gupta D, Rastogi D, Gulrajani M. Naphthoquinone colorants

from Arnebia nobilis Rech. f. Coloration Technol 2012;128:350‑5.

Mitra RN, Das PK. In situ preparation of gold nanoparticles of varying

shape in molecular hydrogel of peptide amphiphiles. J Phys Chem C

Nanomater Interfaces 2008;112:8159‑66.

Gole A, Dash C, Ramakrishnan V, Sainkar S, Mandale A, Rao M, et al.

Pepsin‑gold colloid conjugates: Preparation, characterization, and

enzymatic activity. Langmuir 2001;17:1674‑9.

Whaley K, Burt AD. Inflammation, healing and repair In: MacSween

RM, Whaley K, editors. Muir’s Textbook of Pathology. 13th ed. London:

Arnold; 1996. p. 112‑65.

Cotran RS, Kumar V, Robbins SL, Schoen FJ. Inflammation and repair. In:

Robbins Pathologic Basis of Disease. 5th ed. Pennsylvania: W.B. Saunders

Company; 1994. p. 51‑92.

Mohan H. Inflammation and healing. In: Textbook of Pathology. 5th ed.

New Delhi: Jaypee Brothers; 2005. p. 133‑79.

Rao KJ, Paria S. Green synthesis of silver nanoparticles from aqueous

Aegle marmelos leaf extract. Mater Res Bull 2013;48:628‑34.




DOI: http://dx.doi.org/10.22377/ajp.v8i2.345

Refbacks

  • There are currently no refbacks.