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C18 (250 x 4.6 mm, 5 Î¼m) column using a mobile phase of 0.02 M phosphate buffer solution: acetonitrile (70:30v/v, pH 3.0). The flow rate was 1.0ml/min with detection at 221 nm. The retention time for amlodipine was 2.57 min and for
metoprolol 4.49 min. Amlodipine and metoprolol showed a linear response in the concentration range of 10-110 Î¼g/ml. The correlation co-efficient (â€˜râ€™ value) for amlodipine and metoprolol was 0.9991 and 0.9992, respectively. The results of
analysis have been validated statistically and by recovery studies. The percentage recoveries obtained for amlodipine and metoprolol ranges from 100.04 to 100.57%.
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