Molecular Characterization of Extended Spectrum Β-Lactamase Producing Escherichia Coli Isolated From Urine Samples

Abstract

Aim: The upsurge in multiple antibiotic resistance strains of pathogenic bacteria has become one of the biggest
concern for the past 25 years. Research states that the developments of resistance to multiple drugs are primarily
due to the antibiotic resistant genes acquisition through grouped in multifaceted clusters, transposons, and plasmids.
Materials and Methods: The collected urine samples were polymerase chain reactions (PCR)-screened for
the confirmation of Escherichia coli and the presence of multiple antibiotic resistant markers blaCTX-M coding
the most common resistant phenotypes established in enteric bacteria group. Results and Discussion: During the
present study, pathogenic isolates of extended-spectrum β-lactamase (ESBL) producing E. coli causing urinary
tract infections (UTI) infections from different hospital environments were detected for their virulence gene using
PCR; further, they were examined for antibiotic sensitivity patterns against varied therapeutic drugs available in
the marketplaces. It was found that the antibiotic sensitivity was high for Carbapenems followed by Ofloxacin
and Doxycycline hydrochloride. Moreover, least sensitivity was recorded for Cephalosporin. The present study
found that UTI initiated by Pathogenic ESBL producing E. coli among nosocomial infections which are to be
high among the persons with immunologically suppressed. Conclusion: Drug resistant E. coli may be freely
come across in hospital locations during every day clinical practices and the urologists must act untimely. The
organization of this infection is exceedingly significant for forthcoming research with specific allusion to prevent
of fresh antibiotic resistant pattern.