A Review on High-Throughput NovelAnalytical Techniques for DrugDevelopment and Quantification

Abstract

High-throughput analytical techniques have become indispensable tools in modern pharmaceutical research due to the growing demand for rapid, reliable, and resource-efficient drug quantification methods. Conventional
analytical approaches often struggle to accommodate the increasing complexity of drug molecules, formulation strategies, and large sample volumes generated during drug discovery, development, and quality control. Advances in high-throughput chromatography, spectroscopy, spectrometry, microplate-based platforms, microfluidic systems, automation, and data-driven technologies have significantly transformed analytical workflows, enabling faster analysis without compromising accuracy, precision, or regulatory compliance. This review provides a
comprehensive overview of high-throughput analytical techniques applied to the development of novel methods for the quantification of selected drugs. Emphasis is placed on high-throughput chromatographic, spectroscopic, and spectrometric approaches, along with emerging lab-on-chip technologies, robotic automation, and artificial intelligence-assisted data analysis. The application of these techniques across pharmaceutical and biomedical fields, including drug discovery, bioanalysis, quality control, and clinical research, is critically discussed. Regulatory considerations, validation challenges, and future perspectives are also addressed, highlighting the role of highthroughput
analytical platforms in advancing efficient, reproducible, and sustainable drug quantification strategies.