Physico-Chemical Characterization andAcute Toxicity Study Ethanolic Extract ofParthenium Hysterophorus
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Abstract
Objectives: Parthenium hysterophorus is an invasive herbaceous plant and considered as noxious weed.
Sesqueterpines lactones which are responsible for allergenic nature cause various serious illness ranges from
allergy rhinitis to asthma. Physico-chemical Characterization and acute toxicity study of plant data support to
find suitable solution to overcome the exposures and draw medicinal value of uncontrollable spreading weed.
Materials and Methods: Ethanolic extractof P. hysterophorus purchased from AMSAR Pvt. Ltd., Indore, Madhya
Pradesh, and perform organoleptic characterization, solution stability, powder bulk characterization, extract
microscopy (Labomed Lx 400) at ×40. Phytochemical screening and Infra-red spectrum (Fourier transform infrared
spectroscopy, Shimadzu) determine also perform protein estimation using folin-lowry method and acute toxicity
study on 06 albino rats on three different reported concentrations. Results: The powdered extract bulk density
0.6 g/cm3, Tapped density 0.86 g/cm3 and angle of repose is recorded 38° which reflects the powdered extract flow
falls in fair to passable range. In the screening the main phyto-constituents observed presence of sesqueterpines
lactones, alkaloids carbohydrate, flavnoidsand saponins, in the microscopy study presence of pollens, pollen sacs,
corolla and trichomes. Acute toxicity study of ethanolic extract of P. hysterophorus showcase toxic symptoms
includes Food avoidance, Sedation and minimum movement, Irritations and restlessness observed respective to
dose found lethal dose (LD50) 600 mg/kg in albino rats. Protein estimation observed 90 μg/mL in 10% sample
solution. Conclusion: Characterization of ethanolic extract of P. hysterophorus provide insight related to presence
of allergen protein Par-H-1 and LD50 in albino rats, these data can be use to troubleshoot the management of
disease related to weeds such as seasonal pollen allergies. More detail chemical analysis also requires enhancing
understanding the allergen nature of the weed
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