Immunomodulatory Activity ofIpomoea sepiaria Root Extractin Cyclophosphamide-InducedImmunosuppressed Swiss Mice

Abstract

Aim: The present study aimed to evaluate the immunomodulatory activity of Ipomoea sepiaria root extract
(ISE) in cyclophosphamide-induced immunosuppressed Swiss mice. Materials and Methods: Swiss albino
mice were randomly divided into five groups, including normal control, model control, thymomodulin-treated
group, low-dose ISE group, and high-dose ISE group. Immunosuppression was induced by intraperitoneal
administration of cyclophosphamide (200 mg/kg). Cell-mediated immunity was assessed using delayed-type
hypersensitivity (DTH) response to ovalbumin, while humoral immunity was evaluated by measuring serum
immunoglobulin G (IgG) levels. Hematological parameters, such as total leukocyte count and differential
leukocyte count were analyzed. Cytokine levels, including interleukin-2 and tumor necrosis factor-alpha were
quantified using enzyme-linked immunosorbent assay. Results and Discussion: Treatment with ISE significantly
ameliorated cyclophosphamide-induced immunosuppression. The extract restored DTH response, improved
leukocyte counts, and significantly increased cytokine levels and IgG concentrations compared with the model
control group, indicating enhancement of both cellular and humoral immune responses. Conclusion: The
findings suggest that ISE possesses significant immunostimulatory activity and may serve as a promising natural
immunomodulatory agent for improving immune responses under immunosuppressed conditions.