Spectrophotometric estimation of pioglitazone hydrochloride in tablet dosage form

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Pawan K Basniwal
Prabhat K Srivastava
Deepti Jain

Abstract

Two simple, rapid, and precise methods - linear regression equation (LRE) and standard absorptivity - were developed and validated for the estimation of pioglitazone hydrochloride in tablet dosage form. The maximum absorbance (λmax) of pioglitazone hydrochloride was found to be 269.8 nm in methanol:water:hydrochloric acid (250:250:1). Beer-Lambert law was obeyed in the concentration range of 10-50 μg/ml, and the standard absorptivity was found to be 253.97 dl/g/cm. Both the methods were validated for linearity, accuracy, precision (days, analysts, and instrument variation), and robustness (solvent composition). The numerical values for all parameters lie within the acceptable limits. Pioglitazone hydrochloride was estimated in the range of 99.58-99.97% by LRE method and 100.25-100.75% by standard absorptivity method. At 99% confidence limit, the F-test value for the methods was found to be 1.8767

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How to Cite
Basniwal, P. K., Srivastava, P. K., & Jain, D. (2014). Spectrophotometric estimation of pioglitazone hydrochloride in tablet dosage form. Asian Journal of Pharmaceutics (AJP), 2(4). https://doi.org/10.22377/ajp.v2i4.200
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References

Sweetman SC, editors. In: Martindale: The complete drug reference,

rd ed. Vol. I, USA: Pharmaceutical Press; 2002. p. 353.

Zhog WZ, Williams ME. Simultaneous quantitation of pioglitazone and

its metabolites in human serum by liquid chromatography and solid-

phase extraction. J Pharm Biomed Ana 1996;14:465-73.

Yamashita K, Muarakami H, Okuda T, Motohashi M. High-performance

liquid chromatographic determination of pioglitazone and its

metabolites in human serum and urine. J Chrom 1996;677:141-6.

John-Lin Z, Ji W, Desai-Karieger D, Shum L. Simultaneous determination

of pioglitazone and its two active metabolites in human plasma by

L-MS-MS. J Pharm Biomed Ana 2003;33:101-8.

Kolte BL, Raut BB, Deo AA, Begaol MA, Sinde DB. Simultaneous

high-performance chromatographic determination of piogliatzone

and metformin in pharmaceutical dosage form. J Chrom 2004;42:

-31.

Sane RT, Menon SN, Inamolar S, Mote M, Gundi G. Simultaneous

determination of pioglitazone and glimpiride by high-performance

liquid chromatography. Chromatographia 2004;59:451.

ICH. Text on Validation of Analytical Procedures, International

conference on Harmonization of Technical Requirements for

Registration of Pharmaceutical for Human Use, Geneva: Jul 2000.

ICH. Validation of Analytical Procedure: Methodology, International

Conference on Harmonization of Technical Requirements for

Registration of Pharmaceutical for Human Use, Geneva: Jul 2000.