Reversed phase liquid chromatographic conditions for simultaneous determination of antihypertensive formulations

Main Article Content

Shalini Joshi
Amrita Sharma
MS Rawat
CS Bal

Abstract

Rapid and simple reversed phase chromatographic conditions have been developed to separate pharmacologically
active components of antihypertensive drug formulations (enalapril maleate + amlodipine besilate and
ramipril 1 hydrochlorothiazide) on high-performance liquid chromatography (HPLC) and thin layer chromatography.
The mobile phase consists of methanol–water (60:40 v/v) for reversed-phase thin layer chromatography (RPTLC). Detection
was carried out by iodine vapors. The HPLC method was developed on a C-18 column with detection carried out by a UV
detector at 215 and 220 nm for enalapril maleate 1 amlodipine besilate and ramipril + hydrochlorothiazide, respectively.
The HPLC method was validated using data elements specificity, linearity and range, and accuracy and precision.

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How to Cite
Joshi, S., Sharma, A., Rawat, M., & Bal, C. (2014). Reversed phase liquid chromatographic conditions for simultaneous determination of antihypertensive formulations. Asian Journal of Pharmaceutics (AJP), 3(4). https://doi.org/10.22377/ajp.v3i4.298
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Joshi, et al.: Reversed phase liquid chromatographic conditions for simultaneous determination of antihypertensive formulations