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mucoadhesive polysaccharides extracted from Tamarind seeds (Tamarindus indica) for the sustained delivery of
anticancer drug irinotecan. The drug loaded NPs were prepared by ion gelation method with the isolated polysaccharide by
homogenization followed by lyophilization. The polysaccharides were crossâ€‘linked with sodium alginate in different ratios. The
formulations were optimized using two level factorial design (Design Expert â€‘ 22.214.171.124) using the polysaccharide to alginate
ratio, homogenization time and homogenization speed as independent variables and particle size (PS), drug entrapment
efficiency and cumulative drug release as the dependent variables. The NPs were characterized in terms of PS, entrapment efficiency, drug loading (DL), in vitro drug release and cell viability studies in mice. Stable NPs were obtained with average PS of 405 Â± 25.2 nm. The preparations were homogenous showing polydispersity index of 0.497 Â± 0.02. The formulation showed up to 95.36 Â± 3.1% (w/w) yield showing DL of 1.0 Â± 0.2% (w/w). The entrapment efficiency was found to be
46.56 Â± 1.5% (w/w). In vitro drug release showed initial burst release followed by controlled release pattern showing up
to 60% release in 12 h. The average cell viability was found to be 80% in case of the control group, which was reduced to
36% for NPs treated groups respectively. The Fourier transform infrared studies showed no incompatibility in the formulated
NPs. It may be concluded from the study that tamarind seed polysaccharides may be suitable for formulation of mucoadhesive NPs for better efficacy and sustained delivery of anticancer drug irinotecan with reduced toxicity.
Key words: Homogenization, irinotecan, mucoadhesive, nanoparticles, tamarind
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