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treat oral infections by using Poly (D, L lacticâ€‘coâ€‘glycolic) acid (50:50) as polymer. Microspheres were prepared using
oilâ€‘inâ€‘oil (O/O) and waterâ€‘inâ€‘oilâ€‘inâ€‘water (W/O/W) double emulsion solvent evaporation method. Microspheres prepared
by W/O/W were spherical in shape compared those prepared with O/O method. Thus, the microspheres formulated by
W/O/W method were further evaluated for particle size, morphology, entrapment efficiency, and percent drug release.
Effects of salt addition, polymer concentration on the characteristics of microspheres and tetracycline release profile were
investigated. An increase in polymer concentration decreased drug release and increased entrapment efficiency of drug.
In vitro studies indicated that release of drug from microspheres could be controlled for 10-15 days depending on drug:
Polymer concentration. Formulation E released 99.10% of drug from microspheres in 10 days. Addition of sodium chloride
to outer aqueous phase produced spherical microspheres with smooth surface and also increased entrapment efficiency.
Microspheres were further dispersed in optimized formulation of mucoadhesive in situ gel of Pluronic F127, which acts
as carrier for microspheres. In vivo studies were conducted on patients who underwent molar tooth extraction to check
efficacy of designed formulation.
Key words: Double emulsion, in situ gel, microspheres, molar tooth extraction, poly (D, L lacticâ€‘coâ€‘glycolic) acid, tetracycline hydrochloride
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