Studies on influence of process variables on performance of gliclazide mucoadhesive microcapsules

Main Article Content

Bala Vishnu Priya Mukkala,
Murthy TEGK


Microencapsulation is a unique technique of controlled drug delivery system and is of major importance for effective alteration of drug release required to produce a novel formulation with desired characteristics to overcome the disadvantages of the conventional therapeutic dosage forms. Microcapsules offer efficient absorption and enhanced bioavailability owing to their higher surface area, however, mucoadhesive microcapsules render more intimate contact with
the mucus membrane thereby leading to increase in the gastro intestinal residence time. Gliclazide is an oral hypoglycemic second generation sulfonyl urea, which is useful for a long-term treatment of non-insulin dependent diabetes mellitus. In the present investigation, the gliclazide microcapsules are formulated to control the release rate and improve the absorption across gastrointestinal membrane by employing ionic gelation method.The effect of various process variables such as curing time, stirring speed, stirring time, volume and concentration of curing reagent on entrapment efficiency, and drug release
rate was studied. The microcapsules were evaluated for drug content, encapsulation efficiency, in vitro drug release studies. The optimized formulation was selected based on the entrapment efficiency and drug release rate.The optimized formulation of gliclazide microcapsules were evaluated for rheological properties, moisture content, swelling index, erosion studies,
wall thickness and in vitro wash off test. The microcapsules formulated with 2:1 coat:core ratio by using 150 ml of 0.1M Cacl2 solution as curing reagent, at a stirring speed of 400 rpm for 60 minutes and a curing period of 48 hrs were found to be the optimum formulation. The drug release followed zero order kinetics and was controlled by peppas mechanism. The pharmacodynamic activity of optimized gliclazide microcapsules was conducted by measuring blood glucose levels in healthy albino rabbits. The percentage glucose reduction was calculated and the data was treated statistically. The hypoglycemic activity was extended up to 10 hours in case of microcapsules.


Download data is not yet available.

Article Details

How to Cite
Mukkala, B. V. P., & TEGK, M. (2014). Studies on influence of process variables on performance of gliclazide mucoadhesive microcapsules. Asian Journal of Pharmaceutics (AJP), 5(3).


Chowdary KP, Rao YS. Mucoadhesive microcapsules for controlled drug

delivery. Biol Pharm Bull 2004:27:1717-24.

Hong SS, Lee SH, Lee YJ, Chung SJ, Lee MH, Shim CK. Accelerated

oral absorption of gliclazide in human subjects from a soft gelatin

capsule containing a PEG 400 suspension of gliclazide. J Control Rel


Al-Kassas RS, Al-Gohary OM, Al-Faadhel MM. Controlling of systemic

absorption of gliclazide through incorporation into alginate beads. Int

J Pharm 2007:341:230-7.

Trivedi P, Verma AM, Garud N. Preparation and characterization of

aceclofenac microcapsules. Asian J Pharm 2008:2:110-5.

Lachman L, Lieberman HA, Kanig JL. The theory and practice of

industrial pharmacy. 3rd ed. Mumbai: Varghese Publishing House;

p. 52.

Shabaraya AR, Narayanacharyulu R. Design and evaluation of chitosan

microcapsules of metoprolol tartrate for sustained release. Indian J

Pharm Sci 2003:65:250-2.

Zinutti C, Kedzierewicz F, Hoffman M, Maincent P. Preparation

and characterization of ethylcellulose microcapsules containing

-fluorouracil. J Microencapsul 1994:11:555-63.

Wit, Johannes Bernardus Doshi, Hiteshkumar Anilkant Mulund

Sustained-release formulation of gliclazide. European patent

application, EP 2 181 705 A1. 05.05.2010

Desai KG, Park HJ. Study of gamma-irradiation effects on chitosan

microparticles. Drug Deliv 2006:13:39-50

Si-Nang L, Carlier PF, Delort P, Gazzola J, Lafont D. Determination of

coating thickness of microcapsules and influence upon diffusion. J

Pharm Sci 1973:62:452-5.

Lehr CM, Bowstra JA, Tukker JJ, Junginer HE. Intestinal transit of

bioadhesive microcapsules in an in situ loop in the rat. J Control Release