Novel HPLC analysis of cefadroxil in bulk formulation

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Ravi Shankar Shukla
S Pandey
Rajesh Bargale


A rapid, accurate, and sensitive method has been developed and validated for the quantitative determination of cefadroxil
(first generation) in bulk form. An Inertsil ODS, 4.60 × 250 mm2, 5 μm analytical column was used with an eluting
system consisting of a mixture of phosphate buffer (pH 6.5)-methanol 78-32% (v/v) at a flow-rate 1.5 ml/min. Detection
was performed by UV-Vis detector at 210 nm, resulting in limit of detection of 0.06 ppm for cefadroxil per 20-μl injection.
A linear relationship was observed up to 0.2 ppm for cefadroxil. Analysis time was less than 10 min. The statistical evaluation
of the method was examined by means of within-day repeatability (n = 6) and day-to-day precision (n = 7) and was found to be satisfactory with high accuracy and precision. The method may be applied for the determination of the cefadroxil in
bulk formulation in future.
Key words: Cefadroxil, cephalosporins, HPLC, phosphate buffer


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How to Cite
Shukla, R. S., Pandey, S., & Bargale, R. (2014). Novel HPLC analysis of cefadroxil in bulk formulation. Asian Journal of Pharmaceutics (AJP), 2(2).


Eliopoulos GM, Reiszner E, Wennersten C, Moellering RC Jr. In vitro

activity of BMY-28100, a new oral cephalosporin. Antimicrob Agents

Chemother 1987;31:653-6.

Chin N, Neu HC. In vitro activity of BMY-28100, a new oral cephalosporin.

Antimicrob Agents Chemother 1987;31:480-3.

Yun E, Prince A, McMillin J, Welch L. High-performance liquid

chromatographic separation and electrochemical detection of

cephalosporins J.Chromatogr 1998;B712:145.

El-Gindy A, El Walily A, Bedair M. First-derivative spectrophotometric

and LC determination of cefuroxime and cefadroxil in urine. J Pharm

Biomed Anal 2000;23:341-52.

Gallo-Martinez L, Sevillano-Cabeza A, Campins-Falco P, Bosch-Reig F. A

new derivatization procedure for the determination of cephalexin with

,2-naphthoquinone 4-sulphonate in pharmaceutical and urine samples

using solid-phase extraction cartridges and UV-visible detection, Anal

Chim Acta 1998;370:115.

Jehl F, Birckel P, Monteil H. Hospital routine analysis of penicillins,

third-generation cephalosporins and aztreonam by conventional and high-speed high-performance liquid chromatography. J Chromatogr


Fabre H, Blanchin MD, Kok W. Liquid chromatography with amperometric

detection for the determination of cephalosporins in biological fluids.

Analyst 1988;113:651-5.

Ting S. Reverse-phase liquid chromatographic analysis of cephalosporins.

J Assoc Off Anal Chem 1988;71:1123-30.

Moore CM, Sato K, Katsumata Y. High-performance liquid

chromatographic determination of cephalosporin antibioti cs using

3 mm ID columns. J Chromatogr 1991;539:215-20.

Abdel-Hamid M FSQ spectrophotometric and HPLC analysis of some

cephalosporins in the presence of their alkali-induced degradation

products Farmaco 1998;53:132.

Coran S, Bambagiotti-Alberti M, Giannellini V, Baldi A, Picchioni G, Paoli

F. Development of a densitometric method for the determination of

cephalexin as an alternative to the standard HPLC procedure. J Pharm

Biomed Anal 1998;18:271-4.

Gallo Martínez L, Campíns Falcó P, Sevillano Cabeza A. Comparison

of several methods used for the determination of cephalosporins.

Analysis of cephalexin in pharmaceutical samples. J Pharm Biomed

Anal 2002;29:405-23.

Joshi S. HPLC separation of antibiotics present in formulated and

unformulated samples. J Pharm Biomed Anal 2002;28:795-809.

ICH. Validation of analytical procedures: Methodology. Geneva:

International Conference on Harmonisation, IFPMA; 1996.