In vitro and in vivo evaluation of mefenamic acid and its complexes with β-Cyclodextrin and HP-β-Cyclodextrin

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Deelip V Derle
Mrudula Bele
Nikhil Kasliwal


The objective of this research was to improve solubility, dissolution, and, consequently, bioavailability of mefenamic acid, a poorly water-soluble nonsteroidal anti-inflammatory drug, by complexation with β-Cyclodextrin and
HP-β-Cyclodextrin. The complexes of mefenamic acid with β-Cyclodextrin and HP-β-Cyclodextrin were prepared by kneading method and were characterized and evaluated to study the effect of complexation on dissolution profiles and
in vivo advantage. The complexes were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry studies. Phase solubility studies indicated complex formation with possible stoichiometry of 1:1 and a stability constant of 176 M-1 for β-Cyclodextrin and 103.8 M-1 for HP-β-Cyclodextrin. The characterization studies confirmed the inclusion of mefenamic acid within the nonpolar cavity of β-Cyclodextrin and of HP-β-Cyclodextrin. Remarkable improvement was observed in the in vitro drug release profiles in 0.1-N HCl and pH-6.8 phosphate buffer with
all complexes. Mefenamic acid also showed improvement in the in vivo activity when administered orally to rats as compared
with mefenamic acid per se.


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Derle, D. V., Bele, M., & Kasliwal, N. (2014). In vitro and in vivo evaluation of mefenamic acid and its complexes with β-Cyclodextrin and HP-β-Cyclodextrin. Asian Journal of Pharmaceutics (AJP), 2(1).


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